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KMID : 0360220070480010125
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Journal of the Korean Ophthalmological Society
2007 Volume.48 No. 1 p.125 ~ p.134
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Effect of Amoebicidal Agents on the Human Corneal Keratocytes in Vitro
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Lee Ji-Eun
Lee Jong-Soo
Choi Hee-Young
Yu Hak-Sun
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Abstract
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Purpose: To evaluate the effect of polyhexamethylene biguanide (PHMB) and chlorhexidine on Acanthamoeba cysts and cultured human keratocytes.
Methods: Each well of two-fold diluted PHMB and chlorhexidine were treated on the Acanthamoeba cyst suspension of 5×10(4) cysts/ml for 8, 24, and 48 hours to measure the minimal cysticidal concentration (MCC) of each disinfectant and was exposed to the human corneal keratocytes of 5×10(4) cells/ml for same hours to measure the survival rate of keratocytes. Inverted phase-contrast micrograph and electron microscopy for observing the morphologic changes were evaluated.
Results: MCC of PHMB was 9.42 μg/ml, 5.62 μg/ml, and 2.37 μg/ml, and chlorhexidine was 24.32 μg/ml, 10.02 μg/ml, and 7.02 μg/ml respecitvely in 8, 24, and 48 hours. The survival rate of keratocytes at MCC was 91.7%, 64.6%, and 49.7% in PHMB of which significant decreases were found at 24 and 48 hours, and 95.7%, 90.6%, and 78.1% in chlorhexidine of which significant decrease was only found at 48 hours. The higher the concentration of disinfectants, cysts and keratocytes demonstrated more damaged appearance.
Conclusions: The amoebicidal efficacy of PHMB and chlorhexidine was similar. However, in consideration of toxic effect on keratocytes by disinfectants, chlorhexidine is suggested to be more clinically useful than PHMB. J Korean Ophthalmol Soc 48(1):125-134, 2007
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KEYWORD
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Acanthamoeba, Chlorhexidine, Human corneal keratocyte, Minimal cysticidal concentraion, Polyhexamethylene biguanide
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